Frequently asked questions


Does this system have any safety feature (e.g. automatic gas supply cut off)?

To add a new question Yes. There is a flame detector and a solenoid that will shut off the fuel gas if the flame goes out. Another safety feature is the Level Sensor that ensures the U-Tube is filled before starting. There is a gas/air mixture that is flammable and, if the U-Tube is not filled, can escape outside causing a potential fire hazard.go to app settings and press "Manage Questions" button.

The system comes with gas regulator. Is it for connection to the gas cylinder?

The supplied gas regulator does not go on the gas cylinder but is fixed on the back of the instrument. It will handle the pressure straight from propane, butane, or LPG cylinders (20Bar or less). The user needs to provide a hose connection at the cylinder for attachment to the provided hose. They will just use the shut-off valve on the cylinder to provide or turn off the gas.

How much propane gas is consumed when using the Flame Photometer?

It varies a little between 0.2 - 0.3 L/min.

Can acetylene be used with the BWB Flame Photometer?

No. Acetylene can not be used because it is too hot. It could melt some of the materials and the emissions will be shifted in wavelength outside of where we are measuring.

Is it better to use Propane or natural gas for my flame photometer?

In general, Propane is better than natural gas. The lower caloric content in natural gas will not excite the ions as much so you will lose some of the low end sensitivity. Depending on the nature of your samples, that may or may not be an issue. Once an instrument is configured for natural gas it cannot be used for Propane. It would be possible to convert it back to Propane but that would require going inside the instrument and replacing a few parts. Not a very practical situation. If you absolutely must use natural gas it will probably still work for you. I encourage you to use Propane. The bottles are readily available (I use regular barbeque Propane) and if needed, you can get special Instrument Grade Propane from chemical supply houses. I only mention that depending on your samples and how low you need to measure

What value inlet natural gas pressure is recommended for the BWB XP flame photometer? Our customer has the pressure gas distribution in the range of 1.7 to 2.1 kPa. Is it suitable pressure?

That pressure is right on the threshold of what is needed. If it is closer to 1.7 kPa than 2.1 kPa there might be a problem. I recommend Propane/Butane/LPG if at all possible. Can natural gas be obtained in a bottle? If so, that would allow a higher pressure and we would be certain it will work.

What purity of gas do you recommend for the flame photometer, 99.9%, 99.5% or 99.0%?

The purity of gas depends on how much precision you need, the levels you will be measuring and the environment in which you are using the instrument. Most industrial users do well with regular "barbeque" Propane. We have customers that prefer instrument grade. I would try the regular Propane and see if you get good results before going to the more expensive grades. If you want to use Butane you may need to go to a higher grade from a chemical supply house.

In the manual you suggest that butane is better for Barium Ba readings. What about Sodium Na readings? Do Na readings show increased accuracy in butane vs. propane?

There is no increased accuracy with Sodium using either Propane or Butane.

Gas Requirements: What pressure and flow rate of gas is required for the BWB flame photometer? Can it work off gas cylinders (small 5/10kg canisters) or 'gas containers'? We do have pressurized propane gas lines within the building but if the system was installed into an area where there was no gas supply.

Yes, the BWBXP can be used with cylinders. That is how they are used at BWB and by most of our customers. The pressure of propane in such bottles is a bit less than 20 bar and butane is a bit less than that. The in-built regulator will handle that input. It delivers 37 mbar to the instrument. The pressure supplied should be somewhat above the 37 mbar to ensure a good regulation but I am not sure just how much more it should be. The flow is dependent on the flame setting and usually runs about 0.23 l/min.

The obvious advantage of the BWB Flame Photometer is that it is a small, compact unit, easily transported and fits into a small space. We want to install the unit within a fume hood enclosure with an extract of 0.5 to 1m/s. Would this be sufficient to remove the heat generated from the flame? If the ambient temperature was fluctuating, would the calibration be affected?

Your desire to enclose the instrument in a fume hood should be OK. The amount of heat generated is not as much as hotter spectrophotometer types and that flow rate should be very adequate to remove all the heat. The calibration will be somewhat affected by ambient temperature fluctuations. The amount would be determined by just how much fluctuation there is. However, we have a unique feature we call Calibration Correction. It allows even a multiple point calibration to be easily corrected in less than a minute. This feature can be used not only across the day but over days and weeks. To get a better understanding of this feature please view the video on our website

Can the Flame Photometer be put in a Fume Hood?

It should be OK with a few suggestions: Keep the rate of air flow in the fume hood the same throughout the session. Keep the flow as low as possible to still perform as needed. Do calibrations and sample readings the same while the fume hood is operating

Does BWB Technologies USA require a Water Separator as all of the other Flame Photometer manufacturers do?

We do not use a water separator nor is one needed with our BWB Flame Photometer. Other makers use a much higher air pressure and requirethe water separator. Our compressed air is lower and does not have the issue of water falling out. A test was completed using a humidity chamber and the only way that water came out was to have a steaming bath in the chamber where the compressor was drawing up the visible steam


What does Multi Mode mean and how do we switch on this option?

Multi Mode is a special method of calibration where you can make calibration solutions that contain several ions at the same time and then can calibrate them together. The fastest way to access Multi Mode is to press the Multi Mode key. You can also turn on Multi Mode by going to Calibrations/Calibrate Ion/key 8/Multi Mode. Toggle the Accept key to turn on each ion you want to include. It is especially useful when doing multiple ions at many calibration points because it saves a lot of time.

I made calibration for sodium Na, Potassium K, Calcium Ca. When I switch to View Calibration there is written for K (on the raw, where raw data is shown) Crect’d, what does it mean?

Crect'd stand for Corrected, in this case indicating the calibration was corrected. We have a Calibration Correction function where an original calibration can be corrected for Blank and/or Max. Again. It is a time saver especially for multiple point calibrations. After you have done a calibration and the instrument has either drifted or the flame setting has changed (like when you start it up the next day) you do not need to complete a full re-calibration. Instead complete the following steps: Go to Calibrations/ Edit Calibration Choose which ion (or Multi) you want to correct. The Blank corrects the baseline. The Max is corrected by running the highest calibration concentration only and shifts the rest of the curve. You do not need to do all the other solutions. The correction can be re-corrected or erased.

What is the calibration correction method and an example when using a BWB Flame Photometer?

Example: The client often measures high concentrations of Sodium (Na) and Potassium (K). Generally they are measuring the diluted sample with DI water. The problem is; after calibrated using standard solutions 500ppm and 1000ppm for Na and K on multi mode, measuring the standard solution again to confirm the accuracy, normally it will display a little bit high, about 5% higher than standard solution. The next day, measuring the same standard solution it will display too low, in case of 500ppm, it shows about 400-420ppm. In spite of using the same standard solution, what is the reason the results change during that time? Do we calibrate the instrument every day before measuring the sample? Answer: First, I think your customer needs to dilute even further. I suggest they dilute to 50-100ppm or further down. They should get better results because the Na and K will not interfere with each other so much and it will limit salt build up in the Mixing Chamber and Burner that can cause instability. There are many things that affect the calibration. It can change hourly or throughout the day. The next day it can be off by quite a lot. Recalibration is needed often but we have added a feature to make this situation easy for the user. We call it Calibration Correction. It can be used on both single point and multi point calibration. I will give you an example of how it works. Start by doing a multi point calibration at Blank, 25, 50, 75, and 100ppm. At a time later check the calibration by running the 100ppm standard (the highest concentration standard) and it reads something like 90ppm. Then use the Calibration Correction function. While running the 100ppm standard tell the instrument to correct back to 100ppm. The whole calibration curve inside the instrument is adjusted. There is no need to run all the standards. Here is how to use the Calibration Correction. It can only be used when there is a calibration for that ion. Go to the Calibrations Menu or key the Calibration button.Go to Edit Calibration. Choose which ion you want to edit. Key the number of the ion on the display. There is an option to do Multi correction. You will now see a menu where you can Delete, Correct for Maximum, or Correct for Blank. To correct for Maximum run the highest concentration standard used in the original calibration and key accept. To correct for Blank use the original Blank solution. You have now corrected the calibration. The calibration can be re-corrected many times and the correction can be deleted without deleting the original calibration. With a bit of practice through the menus it becomes very easy to correct the calibration so it can be done as often as needed.

Multi Mode and Calibration Correction on the BWB Flame Photometer:

Does that mean all the calibration points will be corrected automatically on the BWB Flame Photometer, when correcting max only or both correcting blank or max? The Blank is corrected by itself. Does it mean absolutely we cannot correct an individual point between blank to max? You cannot correct one of the points in between. When doing the Max correction all the points, except the Blank, are corrected at once. The "Edit Calibration" menu is where you go to do the calibration correction. First it asks which ion you want to edit. You can choose one or more ions or, if you have a Multi calibration, you can choose to do all of them with one key stroke. Additional answers: How about menu "edit calibration", what would be the effect on doing so?

Calibrating 4 or 5 ions at one time on multimode using a BWB flame photometer and explanation of "drift"

Please confirm the availability of calibration of 4 or 5 ions at one time on multimode? Yes, all five ions can be calibrated in Multi Mode. However, Ca will interfere with Ba and should not be present when calibrating or reading Ba. With regard to correcting calibration mode/ menu, which point is available to correct? (blank or each point on multi point calibration?) Both the Blank and the curve can be corrected but they are corrected independently. When correcting a multi point calibration all the points will be corrected when correcting for the highest concentration. Run your Blank and, if it does not read zero, correct for it by choosing that option. Run your highest calibration point and, if it is not right, correct for it. Please explain the meaning of “drift”. What are the causes of drift? Drift is when conditions have changed since the last calibration or correction. Some reasons for drift are ambient temperature changes throughout the day, atmospheric pressure changes, and humidity of the air changes. Some samples can cause clogging which will show up as drift. The calibration correction function makes it easy to adjust for these without needing to do a full calibration.

The system can do multi-point calibrations. How many points?

Multi-point calibrations can be done with up to 10 points (plus Blank) for each ion on the XP and XP Plus. The BIO and BIO-943 up to 5 points (plus Blank).

When can we use one point and multi-point calibration with a BWB XP flame photometer?

Multi-point calibrations can be used in all cases and are recommended when greater precision is needed. Single-point calibrations should only be done when the concentrations of the samples are at or below those stated in the Operating manual (Section 6.2 - Optimum Measurement Ranges). For example, if measuring Na around 20 ppm it is OK to do a Single-point and a Multi-point could improve the results but may not need it. If the Na samples were up to 80 ppm it would be best to do the Multi-point. When doing a Multi-point and the samples are known to be close to each other try to bracket around that concentration. In our 80 ppm (with a tolerance of +/- 10 ppm) Na example, I would calibrate at 70, 75, 80, 85, and 90 ppm. That will give you very good precision within you tolerance. As many as 10 calibration points can be done with the XP, plus the Blank.

How do I Set the Decimal Point on the BWB XP Flame Photometer? Is it with the Ion Resolution?

Yes, the Ion Resolution is setting the decimal point. There is a maximum of four digits total displayed with a maximum of three decimals. If the reading is from 0 to 9.999 three decimals can be displayed (if wanted), If the reading is from 10.00 to 99.99 only two decimals can be displayed (even if three is chosen), If the reading is from 100.0 to 999.9 only one decimal can be displayed. Above 1000 no decimals can be displayed. It is set at one decimal at the factory and it is recommended to keep it at that setting unless you need to see more decimals as the reading will be more noisy with more decimals showing

Is it possible to convert the results displayed in ppm to µmol/L? Can your flame photometer convert ppm in µmol/L?

Our photometer does not convert between the different concentration units. It only displays what the user wants to use. The display can be changed between ppm, mmol/l, meq/l, and mg/l to reflect to what units the user has calibrated. There is another option called "Units". This is for the situations where the user wants to use other than what is there. They will then need to remember what units they are using. They can change the display to Units (Main Menu, Setup, Ions, Next Calibration) and then calibrate using micro-moles/liter calibration solutions. The instrument will then give results in micro-moles/l

Is it possible to save the calibration values on a BWB XP flame photometer?

Once a calibration is done on an ion it remains until it is either erased by the operator, recalibrated, or adjusted using the Calibration Correction feature. Each ion is independent so these actions can be done to any or all ions. The Calibration Correction is particularly useful so a full calibration does not need to be done very often. The instrument can be corrected over days, weeks, and even months.

Does the BWB flame photometer require a period of time to warm up before I can begin using it?

Yes, all flame photometers require a period of time to reach thermal equilibrium. The exact time will depend on the conditions of the local environment. On average if the ambient is say 20 C and the target is 32 C then warm-up should be achieved in 40 min.

Can either methanol or butanol be used as a diluent for my Flame Photometer?

There are two reasons to not use methanol or butanol: There could be a slight attack on materials. The Butanol could swell the aspiration tubing with prolonged use and could attack the epoxy bond on the nebuliser. The flame characteristics will be changed. This could cause either an enhancement or a depression of the signal.

Could you please explain to me why it is good to use Diluent Concentrate, how does it react?

The Diluent Concentrate is a non-ionic surfactant that is used to lower the surface tension of the Calibration Standards and the Samples to keep the aspiration the same. The more the Calibration Standards and Samples are the same, the better the results. If the Samples are simple salt solutions, the Diluent Concentrate does not need to be done but it will improve the aspiration. If the Samples contain proteins or oils the Diluent Concentrate will help to keep the aspiration the same between the Samples and the Calibration Standards

If we prepare the standard solution strictly following the operation process with a value of 50ppm, does the value show on the screen have any possibility of not showing 50ppm? Should we mix the Standard solution well in advance?

If the instrument is fully warmed up (at least 45 minutes) and calibrated properly with a 50 ppm Standard, it should then read 50 ppm with that same Standard used as a sample. All solutions should be fully mixed and good laboratory practices are needed to achieve good results. Avoiding contamination and ensuring cleanliness are important. Remember to do calibrations and readings with the same technique. Use the same cup size and place the cups in about the same position. Do not hold either with your hands while measuring.


Proper Environment for using the BWB Flame Photometer and tips for stability

Question: I have noticed that even after 30 to 40 minutes the sodium and potassium raw readings are not really stabilizing.The indicator shows a small "Blob" with the raw readings shifting by no more than one unit for each of the ions. For example 63 will flick to 64 then back to 63. Is the instability of the readings caused by specific factors? There is a main road that runs past our Flame Photometer location (less than 10 Meters from the instrument) and during the day the traffic is very heavy. I presume exhaust fumes contain a lot of contaminants which could produce a reading on the FP? There are cigarette smokers in an adjacent office, will this cause interference with the Flame Photometer? Answer: Everything you mention about the environmental situation will affect the stability. Na will be the worst because there is a lot of Na in smoke/dust/exhaust/dog hair. The K is not quite as prevalent in the environment as Na. Li is not at all.

Measuring Samples

We purchased a BWB flame photometer and the results or detection is not correct for Calcium. Our sample is a Fertilizer. This has 50% P2O5 and 10% Nitrogen in the form of Urea and 10% Calcium Oxide. The sample has a pH of 1.75 in 1 % by weight solution. Instead of expected reading of 71 ppm, it will only result to 3 to 5 ppm

The phosphate ion is an extreme contaminant to Ca analysis. There could be some present from the P2O5, either as a contaminant or through unknown reactions. It does not take much to interfere. Is there any way to know if it is present? Another thing I see is the pH is very low. You might try to raise the pH but not enough to precipitate out the Calcium Hydroxide. Try Ammonia solution to not add other ions. Let me know if this helps.

I tried to increase the pH of solution to analyse and it has no effect by adding Ammonia Solution. Different pH is adjusted and the reading is the same from pH 2 to 8 and no precipitates as above 9 is having precipitates, solution became hazy. The reading of my sample is expected to be around 34 ppm. I do standard calibration of 100 ppm in single point. I also did 2 point calibration and reading is still low at 5 ppm. We always analyse the content of P2O5 presence in our sample and the presence of P2O5 in sample is ranging from 85 – 120 ppm P2O5

I was hoping it would at least show an improvement. It is going to require some research to figure this out. The only way I can think of is to make samples that omit each component and test to see which one is supplying the interfering species. These should go through the normal manufacturing processes. Include any "inert" ingredients, like binders or flow agents, which might be present.

I have made a test of which I reduce the pH of standard by adding Urea Phosphate crystals to make the pH of the standards became 2.75. The result is the same as expected with the samples but I am not sure if I am following the correct procedure. I add Urea Phosphate crystals to Ca calibration standard and then it gave good results in the expected range. Is it OK to add to the Ca Calibration standard?

Good to hear that you have good results now. Yes, it is OK to add to the calibration standard, within reason. What you have done we call a matrix correction. By adding the species that is causing the interference it "reacts" the same way as in the sample. Now, when doing the calibration, you tell the instrument that you are calibrating at 100ppm but there is a fraction that is not available for measurement because of the interferent, so it is seeing less than the 100ppm. The instrument takes the lower signal and calls it 100ppm. When the sample is run it too has the same fraction that is not available so it will have the same lowering of the signal and the instrument reports the results that are now corrected for the interferent. For the best results the interfering species should be close to the same concentration as in the sample. There is a slight interference on Ca from Na, depending on the concentration. You might want to correct for that by adding the appropriate amount of Na to the Ca Standard.

Our samples include serum, urine, sweat, drink (soda/milk). Please confirm they can be used with your flame photometer.

These items certainly can be analyzed with flame photometry. In fact they were some of the first materials analyzed when it was invented over 70 years ago. These samples will need some form of preparation (dilution, de-proteinizing, etc.) prior to running through the instrument. Our literature is rife with various methods of preparation and we have some of them in our Guide to Flame Photometry. You need to determine which preparations are needed for your samples.

How do I ensure precision results?

There are some things to ensure precision results: Clean the Mixing Chamber and Burner parts often. Keep the samples diluted to 100ppm or less. Do a multi-point calibration bracketing the expected concentration of the sample. Use the same techniques to calibrate and measure samples. Use the same size sample cups filled to the same level and place them in the same spot on the spill tray. Do not hold by hand.

Brine sample preparation and tips for measuring Barium; underground wastewater with the BWB Flame Photometer

Question and application specifics: The fluid is a brine wastewater from natural sources (deep underground). There are two different streams with main components shown below. Stream #1 - typical levels of barium are 5,000-15,000 mg/l. There is also the following but range can vary beyond numbers below: Ca: 5,000-40,000 mg/l, Na: 10,000-60,000 mg/l, Sr: 1,000-8,000 mg/l, Cl: 20,000-200,000 mg/l, and others typically in a brine water - carbonates, potassium, iron. May also have low levels of organics Will have high TDS, can be up to 300,000 mg/L. Stream #2 - Similar to above but Barium typically 1 - 200 mg/l. Answer These brine samples are a bit of a problem. They cannot be run directily through the flame photometer. The salt content is too high and salt deposits will form in the Mixing Chamber and Burner in a matter of minutes. Once they are there, they will throw off particles that will create a very noisy signal. Another reason is the interferences are huge and make it very difficult to compensate. Normally, higher salt concentrations are diluted down to relieve those situations. However, with these, to get the salts down enough, the Ba will be diluted too far to get a reading. Are you only interested in Ba and not the other ions? I think the only way to approach this is to precipitate the Ba (as the sulphate) away from the rest of the salts and redissolve (with ammonia EDTA) and then analyze. The Barium Sulphate is 1000 times less soluble than the Strontium Sulphate and 100,000 times less than Calcium Sulphate so they should not be too much of a problem as long as there is not much of an excess sulphate added. This plan will help with Stream #2. The Ba in it is very low but can be magnified through the precipitation/re-dissolution process. For example, if it is 100mg/l in the sample and you precipitate from one liter you will get 100mg of Ba. Re-dissolve into 250ml will give you 100mg/250ml or 400mg/l, four times as concentrated. Remember to back calculate for the original sample concentration. If you want to measure the other ions remaining after precipitating the Ba the sample can be diluted now and measured directly. I would recommend a 1,000:1 dilution factor.

Determination of lithium in blood serum using flame photometry

I want to determine the level of lithium in blood serum using flame photometry, please advise whether you have calibrants in units for biochemistry (mmoles/l, etc) with the BWB XP flame photometer? Our XP standards are 10,000ppm. We have mmol/l standards for the BWB BIO and BIO-943flame photometers.

How is mmol/l converted into ppm?

To convert into mmol/l divide ppm by the atomic mass of the ion in question (6.94 for Li). When diluting the Standard Concentrates in ppm to mmol/l be sure to do so at 20 Degrees C

Can we measure K and Na in bio diesel using the BWB-XP?

It depends on what stage of production you want to measure. The actual biodiesel cannot be run through the instrument. It will attack several of the components and will change the flame temperature. It is usually the waste portion that is measured to make sure the Na and K compounds are fully washed out. Many of these are soap-like compounds and are washed out with water. As long as it is toward the last of the wash so there is not much "soap" it should be OK. The first of the wash would have too much "soap" and would foam inside the Mixing Chamber causing erratic readings.

Measuring Sodium and Potassium in Cheese with the BWB XP; comparison of flame photometry with ICP and AAS

Question I am most interested in sodium ion quantization in semi-solid or solid samples such as cheese. Secondarily I am interested in potassium. The concentration is relatively high in the solid, 1000’s of ppm, but in solution that will obviously be dependent on sample preparation. Sample preparation is key to our interest in flame photometry. While I understand that the measurement itself is straightforward, what sort of sample preparation would you expect for these samples? Is it similar to ICP? Is acid digest required? Answer From what I have seen the most common method for these types of samples is to ash and dissolve with acid. The resulting solution should be diluted to keep the Na and K below 100 ppm. Also, be sure to filter any particulates that are usually present with ashing/dissolving methods. You will need to find a filter system that will not contaminate with Na and K. Regardless of the sample preparation, once you have the sample flame photometry has advantages over other instrumentation, as long as you are only interested in the few elements it measures. Properly diluted, the interferences from other elements literally go away. It is easier to use than other instruments. No adjustments of fuel and oxygenate gases, no slit adjustments nor wavelength choices. The operator need not be a highly educated (paid) spectroscopist. Much more affordable to purchase and fast to get final results. This translates into a more effective cost/sample ratio.

Producing standards for KCL measuring; concentrations and dilutions levels

Queston: I am considering making new standards for the photometer to analyze our finished product. Typically we measure the NaCl and other constituents (MgCl2 & insoluble) and through elimination of them, determine the 95-99% pure KCl we have produced. When building these standards in the past we have made up varying solutions standards of NaCl and allowing for the matrix of the others, have actually used about a 4000 ppm combined salt (NaCl, KCl & MgCl) solution. Do you think this is too high? I'd started to examine the possibility of reducing our standards down to 1000 ppm salts in total. It was my understanding that even though the NaCl content was below the 1000 ppm on our present standards the excess KCL/MgCl2 added for matrix effects may be impacting the proper reading of the Na. Would this be advisable? As a practical matter I think you should reduce the Standards and samples even further. Closer to 100-400 ppm. Even lower would be better. Can you go to 10-40 ppm? I say this for two reasons. The more diluted samples would help to mitigate the effects of the other ions and you may not need to do the matrix. At such high concentrations the salts tend to crust on the inside surfaces of the Mixing Chamber and Burner parts. They then start coming off and entering the flame causing wild readings. These parts will need to be cleaned often. Even as often as every hour or sooner! Not very practical. When in doubt, dilute it out.

Is the BWB Flame Photometer suitable for use with sample concentrations containing 0.1ml per liter sulphuric acid?

That level of sulphuric acid will be OK to use

Is it possible to use the BWB Flame Photometer with HF in the sample?

Even at low concentrations HF acid should not be used. It will attack the stainless steel capillary on the Nebuliser and Burner, the silicone U-Tube/Waste tubing, the Level Sensor, and the glass Drain Cup. It might take some time to cause a failure of these components at low concentrations but it will happen eventually. The use of HF will void the warranty

Is the BWB XP instrument suitable for Measuring Lithium and Barium in iron oxide?

This application has some difficult issues. First is the wide range they are looking at. It is not practical to run samples that are high in salt concentrations. More than a few hundred ppm and the salts deposit on the insides of the mixing chamber and burner. They will then come off erratically and the readings will be very unstable. Therefore, the mixing chamber and burner will need cleaning often. Keep the concentrations at 100ppm or below. The lower levels will be good but the higher levels will be a problem. Do not even think about running 20000ppm! If they know their samples are low they may not need to dilute them and if they know they are high they can dilute them accordingly. If they have no idea what the concentration is before they start it will be much more difficult. The next issue is that almost everything in this system will interfere with each other at higher concentrations. Another reason to dilute the samples. Lithium- Iron at dilute levels (30ppm) starts to depress the Li response. Barium also has an effect on Li but is not too bad under 300ppm. The acids will also have an effect. Keep hydrochloric under 0.03 M and the sulphuric under 0.1M (when the samples are diluted). Barium- Iron has a very strong effect and should be absent. I am not sure about the effect of Li. The sulphuric acid will depress the response but I do not know how much. The hydrochloric is good up to 0.1 M. Since they are measuring in iron oxide the iron will be too high even if they dilute. To get the iron down to where it will not interfere, the Ba and Li will be too low. Therefore, they will probably need to precipitate out the iron. It is possible that they are already doing this and it will not be a problem. An alternative is to remove the Ba (as a precipitate) and Li (using ion exchange resins) from the samples, re-dissolve and then measure them separately. This will keep them all apart and they will not interfere so the matrix compensation will not need to be done. The interferences between the Ba and Li can be dealt with through matrix compensation techniques. That is, when making up calibration standards for each ion the other ion needs to be at the same concentration expected in the sample. For example, if the Ba is 100ppm and the Li is 20ppm all the Ba standards will have 20ppm Li and all the Li standards will have 100ppm Ba. If the acids are a bit high they can similarly be compensated

Sample measurement of polymer samples. The sample is diluted liquid condition of polymer with NaOH solution and the concentration of it is about 24-25%.The client wants to measure the concentration of Na of this sample. According to the customer, it is difficult to measure Na by reason of high concentration; it can be diluted with DI water.

The sample will need to be diluted. At 25% NaOH that is 250g/l or 144g/l of Na. Doing a 1000:1 dilution (1ml diluted to 1 liter) gives 144mg/l Na. This would be the minimum dilution needed. It would be better to dilute an additional 10 times (10,000:1 or 0.1ml diluted to 1 liter) to give 14.4mg/l. That will put them in the range where the Na is very well behaved with little interferences. There could be a problem with the polymer portion. If it does not precipitate out when diluting it could clog the nebuliser or coat the mixing chamber. Both would then need to be cleaned often

Relationship of Ba and Ca using a BWB flame photometer

Ba poses issues. There cannot be any Ca present when measuring Ba. The other ions will interfere with each other at concentrations above 100ppm. The samples will need to be diluted to avoid these interferences.

Calcium measurement in Chemical Fertilizers containing CaCO3 or CaF2 or CaSO4 using flame photometry

The measurement of Ca can be difficult in many types of samples and fertilizer is one of them. The problem is that all of the anions will interfere with the measurement and the Ca needs to be separated from them. This will entail quite a bit of chemical techniques. There are two main types of separations to get the Ca out. If the sample is a solid the use of a Lithium Acetate solution to extract the Ca out has been successful. For liquid samples the Ca is precipitated as the oxalate and re-dissolved with perchloric acid. In our "A Guide to Flame Photometer Analysis", there are a few examples of how to measure Ca. Although those methods are not specific for fertilizers, you can try them anyway. The techniques and chemicals would be the same.

BWB-XP and UrineCan the BWB-XP measure urine samples up to 200 mmol/l?

Question Can the BWB-XP measure urine samples up to 200 mmol/l? Is there something known about the linearity of sodium urine in the range 10 - 200 mmol/l? In your biochemical applications you describe only the measurement in serum (120-160 mmol/l). Do you have a manual for urine? Although urine is not the same as serum it can be thought of as the same when using the flame photometer. It is actually easier because it does not have all the proteins and blood cells found in serum. It does need to be diluted by at least 100:1. The response of Na at the high levels in urine will not be linear. This is one reason to dilute, to get it in the range where the Na is much more linear. Another reason is to limit the interference with other ions, particularly K. Tie that with our multiple calibration points feature and you should get very good results. There is not a specific manual for urine.

How do I convert ppm to mmol/l?

To convert use the following formulas: ppm = A x mmol/l mmol/l = ppm/A A = atomic mass of the ion.

How do I use the BWB Flame Photometer to make Calcium (Ca) and Sodium (Na) measurements in animal feeds?

I see no serious problems measuring Na. The Na will be re-dissolved and can be measured in the normal manner. It will not be affected very much by the other ions at their expected concentrations. For Ca, the proteins would be burned off with the ashing method. I am not sure that the phosphates will be gone with ashing. If not, I have a reference to use EDTA to chelate the Ca to "protect" it from the phosphate. I have not tried this yet to know if it works. I do see a slight problem. If the levels of the other ions are present in the listed table (page 4 of the digestion procedure 3.0-E) the Cu, Fe, Mn, and Zn will lower the Ca results. You might be able to compensate by adding these elements to the Calibration Standards (and Blank) if you know they are present in the sample. If they are in the calibration solutions but not in the sample, then the Ca reading will be high. How will they know if they are present? If they are using another method to determine them it should be done first to see if they are present and then they can compensate. They will need to do some investigating to see how well it will work. The only other issue is the digestion process uses nitric and hydrochloric acid mixture. I know that most of the acid will be used up in the digestion but if not it could attack the stainless steel needle in our nebuliser. Something to be aware of.

Is the BWB flame photometer suitable for measuring all 5 ions in water samples in natural environments?

Yes, the BWB-XP is ideally suited to be used with waters from lakes, streams, rivers, and lagoons. Depending on the concentrations of the ions the samples will probably need to be diluted for the measurement and the reading then multiplied by the dilution factor to get the final result. The ranges for each ion are: Na= 0-1000ppm, K= 0-1000ppm, Li= 0-1000ppm, Ca= 10-1000ppm, Ba= 30-3000ppm However, we do not recommend that routine measurements be used at the higher concentrations. For best results the samples should be diluted so they are around 100ppm or less

What does the term 'Matrix" mean relative to the BWB Flame Photometer measuring system?

Matrix is the set of all the species that are present. Some of them we want to measure, some of them interfere with what we want to measure, and some of them are in the background that may or may not affect our results. For example, wine samples have alcohol in them. We know that alcohol will affect the flame which in turn affects the results so we want to add alcohol in our standards to try to get the standards to have a similar matrix as a sample. Now, the wine also has other things in it like colorants, flavors, and bits of grape peels. Those would be much harder to add in the correct amount and I'm not sure that they would've interfered very much anyway so I choose to not add them to my standards matrix

When do we need the Diluent Concentrate? Previously we've used standards with ion exchanged water of good quality. Is this ok with the BWB-XP?

The Diluent Concentrate does not need to be used with all samples. It is a non-ionic surfactant that is used to adjust the surface tension. You may see a slight increase in signal because the drops in the mist will be smaller so more make it to the flame. Its real use is when samples have materials that affect the surface tension, like alcohols, proteins, oils, or fats. Using the Diluent Concentrate in the samples and standards will help to make the surface tension the same with both to give better results. If the samples are simple salt solutions it is not needed at all. It is very important to use good DI water and I see that you are doing so with an exchange resin bed.

How do you properly detect Sodium (Na) and Potassium (K) in blood serum samples? The sample concentrate which user is detecting is below: Na : 3218.39 ± 34.48ppm (equals to 140.0 ± 1.5mmol/L). K : 196.47 ± 5.88ppm (equals to 5.01 ± 0.15mmol/L). Please confirm that the sample above could be detected by BWB-XP.

These appear to be blood serum samples. Yes, they can be detected easily by the BWB-XP. However, they will need to be diluted. Most users dilute by 100:1. Therefore, the standards will need to be made to the diluted values to calibrate. Before calibrating, set the units to mmol/l under Setup/Ions/Next Calibration. There are two options that can be done with the calibration at this point. Use the diluted values (1.40 mmol/l, 0.051mmol/l) and remember to multiply by 100 to get the final results. Tell the instrument that the diluted standards are the concentrations before diluting. Then, they can read the final results directly. The first option will be more precise. The second option is easier and will probably give results within the tolerance they want.

Is it possible for your flame photometer to measure continuously by aspirating samples from a pipe?

First, it depends on the nature of the sample. If the concentrations are more than about 100ppm they would want to dilute before aspirating. Higher concentrations of salts will form deposits inside the mixing chamber and burner. They would need to be cleaned often which would cause them to take the instrument off-line. That could be as often as every hour at 1000ppm. Another issue is the environment where they would situate the instrument. Process facilities are generally dirtier than laboratories. Any dust or smoke in the air will be drawn in and cause instability of the readings. Temperature is also an issue. Will the ambient temperature change during calibrations and readings? If so, that could further muddle up the situation. We have an option of a Collection Cup. The sample is pumped to the collection cup where it is aspirated into the instrument.

How do I measure Sodium Na in sewage water using flame photometry?

In general, the BWB-XP flame photometer can be used for sewage water. However, there are some issues that will need to be dealt with. Sewage water will have many unknown contents that can change hourly. Many of these will interfere with measurements. To limit the interferences the samples will need to be diluted. I recommend diluting until the Na is at 10ppm or less if possible. This may not give a high level of precision so the customer will need to develop a method that works for them. All solids need to be settled/filtered out

What is the Flame Photometry Method for analyzing Ca in fertilizer with Phosphates present in the sample with a BWB Flame Photometer? Our samples are NPK + Ca Fertilizer and our Ca does not give the correct reading because of the interference of Phosphate present in the sample. What is the method for analyzing Ca using the BWB XP flame photometer with this type of sample?

Any phosphate present will interfere. So either the Ca is precipitated out and re-dissolved separately or the phosphate is "tied up" so it isn't available to react with the Ca. (What happens is the phosphate and Ca form a complex that needs a different temperature flame. The Ca is no longer "available" as an element.) Here are some options. Precipitate the Ca as the oxalate and redissolve with perchloric acid. See Methods 14 and 15 in our Guide to Flame Photometry. Remove the phosphate using anion-exchange resins. (Also works on sulphate.) There are many different kinds of resins so they will need to investigate which will work and follow the manufacturers' instructions. Protect the Ca using EDTA as a chelate. All I have is a comment "large amounts of EDTA in strong KOH solutions". Protect the Ca with 1% by weight dextrose. This did not restore the full emissivity of the Ca. 5. Add lanthanum at 5 times the molar content of Ca (after dilution).

Is it possible to measure to measure a concentration of Potassium in KCN (Potassium Cyanide) and KOH (Potassium Hydroxide) solution?

I do not see any reason that this would not be able to be measured. It has the high pH to keep it from forming cyanide gas. However, I would recommend you use a fume hood over the chimney exhaust in case some cyanide gas forms in the flame. If so, you not only do not want to breathe it, there could be some corrosion in the chimney over time.

What matrices can be analyzed? Would the presence of heavy metals (such as uranium) interfere with the analysis? Have you analyzed Lanthanide series elements such as Gadolinium? The BWB is a low temperature flame emission spectrometer. As such, it only measures Na, K, Li, Ca,

The BWB is a low temperature flame emission spectrometer. As such, it only measures Na, K, Li, Ca, and Ba. That is one of the main advantages for most users, as long as these are the only elements one is interested in. Most of the other elements do not interfere making the analysis much easier. The Lanthanides cannot be done.

In order to ensure sampling precision, can we use transfer pipette?

Yes. Whatever method and equipment used the result will depend on the tolerance of the equipment and technique. It is best to use the same methods of dilution on both calibration standards and samples. Be aware of contamination.

How do you prepare Ba standards which also contain ions? As an example: 50ppm, 70ppm, 80ppm, 90ppm, 10ppm Ba standards which also have Na 30ppm, K, 30ppm, Ca 20ppm.

There are two ways to do this, one is harder but it will be more exact while the easier way will not be so exact but still may be good enough for your purposes. I will use your example. Remember that the Standard Concentrates we supply are in mg/l and mg/l = ppm only at 20 Degrees C. I will assume everything is at 20 C. The hard way: Each Ba Standard will need all the concentrates added in their correct proportions. That is, for the 50ppm add 0.5ml Ba, 0.3mlNa, 0.3ml K, 0.2ml Ca and dilute to 100 ml total with DI water. For the 60ppm add 0.6ml Ba, 0.3ml Na, 0.3ml K, 0.2ml Ca and dilute to100ml. Continue in the same manner with the others. The easier way: Make a large batch (1 liter) of a diluent solution that is 30ppm Na (3ml), 30ppm K (3ml), 20ppm Ca (2ml) diluted to1000ml with DI water. Let us call this solution 30/30/20. Then use this 30/30/20 solution to make each Ba Standard. That is, for the 50ppm add 0.5ml Ba and dilute with the 30/30/20 solution to 100ml. Similar with the others. This method will have the background ions vary by 5-10%. Most of the time that will be OK. Another reason to go with the easier way is when compensating for background ions, like in this situation, it is best to use the 30/30/20 solution as the Blank. So, it would need to be made anyway. If the 5-10% variation is OK it saves a good bit of work. I see a possible problem with what you have here. The Ca will interfere with the Ba. That is, the Ca spectrum has a component where the Ba is measured. The Ca signal may be larger than the Ba signal and it will not be seen. If your flame is maximized for Ba the Ca signal may be lessened.

How do I dilute the BWB calibrating solutions?

We supply 10,000 mg/l calibration solutions that can be made into these. For all these solutions the ions in question are all single valence so mEq/l is the same as mmol/l. To convert mmol/l to mg/l, multiply by the atomic mass. For example, the Li=15mmol/l (same as 15mEq/l) is multiplied by 6.94g/mole giving 104.1 mg/l. To make this from our solutions it is easiest to use the equation V1xC1=V2xC2. If you want 500 ml of 104.1 mg/l that would be 0.500 liter x 104.1 mg/l = X x 10,000 mg/l. Solve for X = 0.0052 liter. So take 5.2 ml of the 10,000 mg/l solution and dilute to 500 ml.

How do I measure Sodium Na concentrations and dilutions using a flame photometer? My greater question was if the output dilution rate of 1:200 was in the normal range of the BWB instrument. Our solutions are between 2 and 170 mmol/L in Sodium concentration. After the incoming Na concentrations to the photometer are between 0.01 and 0.85 mmol/L. If I have done my math correctly this would be a ppm concentration range of 0.23 to 19.6. I believe this is within the BWB photometer's specifications. I do possibly have the option to dilute at a rate of only 1:50 if need be.

A 200:1 dilution is very good for the BWB-XP. Your calculations are correct and you will be in the best range for measuring Na with a single point calibration

How do I measure Na Sodium in mouse urine using flame photometry? I am interested in setting my BWB flame photometer to most accurately measure sodium in mouse urine. I was going to set up a multipoint calibration with different concentrations of sodium, and I read that the other chemicals in the solution could alter my calibration curve i.e. urea. What is the best way to calibrate for sodium in mouse urine?

The key to most biological samples is to dilute sufficiently to lower the interferences. If the Na in mouse urine is ~200meq/l I would dilute by at least 200:1. The higher the Na concentration the more dilution. Get it so it is around 1meq/l or less. (Of course, multiply the reading by the dilution factor to get the original concentration.) You are correct to do a multiple point calibration, even at lower concentrations, to get the best results. To help mitigate the action of urea you can do matrix compensation techniques. When making your calibration standards, add urea to your diluent solution at a concentration close to what the urea will be in the diluted samples. With urea at the same concentration in all the standards and test samples it will have the same effect on them all and you will be "compensated" for its presence. Urine can have other chemicals, like proteins, so it will help to make your diluent solutions with the Diluent Concentrate (surfactant). Also, to lessen build up of proteins, urea, etc., use a solution containing the Decon 90 often to clean out the Nebuliser and mixing Chamber, especially after you are done using for the day. Run it for about 20 minutes before shutting down the flame

Minimum sample volumes for a BWB flame photometer We have a customer who asks about the minimum sample volume enough to introduce in the instrument for good measurements; could you please help me? This customer is user using between 100-200 µl. Concentration of this samples is around: Sodium Na: 140meq/l, Potassium K: 5 meq/l, Lithium Li: 0,6 meq/l.

Our flame photometer uses about 1-2 ml of final sample to get a reading. That is 10x what your customer is doing now. However, their concentrations of Na and K will need to be diluted for best results. The Na should be diluted by 100x or 200x. The K can be diluted by 10x. Both of these will bring the final volume where a measurement can be made. The Li will be a bit low concentration at a 10x dilution but may still be okay.

What is the minimum amount of sample for BWB-XP? We sometimes receive inquiries from end users in pharmaceutical field who use automatic flame photometer made by Instrumentation Laboratory(IL) in Italy. The one specific feature of IL flame photometer is measurement of particle sample (e.g the minimum is 20 μl). Then could you please advise us, 1. How much amount of sample is the minimum for BWB-XP? 2. The idea to measure small amount of sample?

We are familiar with the Instrumentation Laboratory instrument. The pharmaceutical use of the IL is for measuring blood plasma and urine. These samples need to be diluted by 100 to 1. Therefore, a 20 µL sample then becomes 2000 µL or 2 mL. We aspirate at about 3 mL per minute. Most readings stabilize within about 15 to 20 seconds so 2 mL can be measured using the BWB. Almost all the time, samples will need to be diluted. How much will depend on the actual sample. Even for industrial users with valuable samples will probably need to dilute also. Always keep in mind that flame photometry works best at lower concentrations. There is less self absorbance and interferences are minimized.

Model Selection

What is the difference between the XP, XP Plus and BIO flame photometers?

The main differences are in the calibration curves. The BWB-XP and BWB-XP Plus can be calibrated up to 10 points (plus Blank) per ion. Once calibrated, it will hold that calibration until it is erased or corrected by the user. The calibration can be accessed by the operator at any time. The XP plus has an additional feature the XP does not. It has an Internal Reference System where the operator has a choice to use either Li or Cs and an internal standard. It will make adjustments for small changes due to flame conditions or drifting. In order to accommodate this, the Ba has been removed. For the BWB-BIO there are a total of four curves that can be calibrated up to 5 points (plus Blank) for each ion. These curves are first split between Serum and Urine. Thye are each further split between Na/K and Li/Ca. The Na/K and Li/Ca each need different dilution factors for proper measurement. The operator cannot access them without a special administration code. The Calibration Correction then becomes their only way to calibrate. Our Calibration Correction feature allows the user to adjust this original calibration curve to account for differences in flame settings with just one calibration standard solution (the highest one used in the original calibration). Many users measuring biological samples do not want operators access to change protocols. The BIO also has the Internal Reference System. Our most recent addition is the BWB-BIO-943. This was meant for users who are familiar with the Instrumentation Laboratories 943 flame photometer. It has two calibration curves, the Serum and Urine with Li enhanced so it can be included with the Na/K. Ca has been eliminated.

What is the main difference between flame photometer and Atomic Absorption?

There are quite a few differences between FP and AA spectrometry. Both introduce the ions into the flame where they are put in an excited state. With FP, as the excited state "relaxes" it emits light of a specific color that is then detected. The more light detected the more ion is in the sample. With AA, the excited state will absorb certain colors. A white light containing all colors is passed through the flame and the excited ions will absorb certain colors. The degree of absence of those colors is a measure of the concentration in the sample

Which is the best model for drinking water analysis?

Drinking water does not have high levels of dissolved salts so should be easy to measure. An XP will work fine but I would recommend the XP Plus if they do not mind using the Internal Reference, meaning needing to add either Li or Cs. It will give a bit better results. It really depends on if they are price sensitive and the lower cost of the XP will be too high.

Is it possible to use the BWB Flame Photometer as opposed to ISE Analyzer?

The BWB flame photometer can be used with biological samples. Flame Photometry was the original method used back in the 1960's when they went from wet methods to instrumentation. Most often the use is for Na, K, and, sometimes, Li. Ca measurements are problematic because the samples require the Ca to be extracted away from the rest of the constituents


What is the range of detection (min to max) for Na, K, Li, Ca, Ba?

The limits of detection (where the signal is not distinguishable from Blank) are: Sodium Na= 0.02ppm Potassium K= 0.02ppm Lithium Li= 0.05ppm Calcium Ca= 1.0 ppm Barium Ba= 10ppm The maximum levels, for practical reasons, should not go much above 100ppm. Experience has shown that high levels will require cleaning of the Mixing Chamber and Burner Tube often. At 1000ppm they might need to be cleaned every hour. This is one of the main reasons that dilution of samples needs to be done.

What is the gas flow rate? Fixed or varied?

The air flow rate is fixed. The user can not adjust.The gas flow rate is adjustable to tune the flame height. It is about 0.4 liters per minute.

Are the calibration solutions delivered with calibration certification?

Yes, the calibration standards are provided with Certificates of Analysis and Safety Data Sheets

What are the limits of detection for Sodium (Na) and Potassium (K)?

Na : 0.02ppm = 8.7 x 10-4 mmol/LK : 0.02ppm = 5.1 x 10-4 mmol/L. Note: These limits of detection are in the best of circumstances. Not all users will be able to achieve them

What is the measurement accuracy for each of the 5 elements that the flame photometer detects?

Accuracy is dependent on many factors so it is difficult to give an exact number. During final testing when we build them we look for 1% or better. This is with simple salt solutions which are easier than serum. Most users with these samples are happy to achieve within 5% which they can do well with the BWB-XP.

What is the accuracy of analysis with the BWB XP Flame Photometer?

It is difficult giving you a definite number. The results are dependent on many factors like the nature of the samples and operator technique. With simple salts diluted to less than 100ppm we routinely get within 1%. Most samples are not simple salt solutions and have many other components that can complicate the measurement. First, there are two things to know before starting. 1. We provide standards at a nominal value of 10,000 mg/l. They are not exactly 10,000 mg/l but we include the Certificate of Analysis that give you the tested value of each batch. For example, the actual value may be 9,980 mg/l. This is the number that should be used to be the most accurate. For my discussions here I will use 10,000 mg/l for ease of use. 2. Again, to be the most accurate all solutions and volumetric ware should be at 20 degrees Celsius. Note that mg/l is the same as ppm only at 20 degrees. Slight deviations from 20 degrees will not throw off the results by much but it is much more difficult to factor in. Most users do not need to be that precise and, most of the time, flame photometry is only good to three significant figures or less. The general formula to use when diluting is: (Concentration 1) x (Volume 1) = (Concentration 2) x (Volume 2).So, if I start with a 10,000mg/l solution and want to make 100ml of a 100mg/l solution I set up my formula as:(10,000mg/l) x (X) = (100mg/l) x (100ml)Solve for X = 1ml. This means that taking 1ml of the 10,000mg/l and dilute it to 100ml will give me a 100mg/l solution. Remember the actual value can be different so, from my example above, you would get a 99.8mg/l solution. If you wanted an exact 100mg/l solution you would use 9,980 mg/l in the formula and you would need to measure out 1.002ml. Rather than trying to measure 1.002ml, when the instrument is calibrated you can enter 99.8

How do I make a calibration curve using graphing paper?

Start with graph paper (a grid layout). Along the bottom axis indicate your concentrations of each of the calibration solutions, keeping them properly spaced. For example, Blank (0ppm), 25ppm, 50ppm, and 75ppm would be equally spaced at whatever distance you choose. Along the vertical axis you will put the Raw readings. First find out what they are and write them down so you know how to space them. When you look at the Raw readings they can get quite large and you will see them changing. It is best to round them off to the first three or four numbers. For example, 31,245 would be rounded to 31,200 and 31,278 would be 31,300. Use these set of numbers to space your indications on the axis keeping the proportions between them. Find the intersections of each concentration and its Raw reading and put a dot there. When all of them are done draw a smooth curve connecting the dots as best you can. You do not need to go exactly through each dot. The best curve you can draw that is smooth and gets as close to all the dots as you can. When reading a sample get the Raw and find it on your vertical axis. Go over level until you intersect the curve. At that intersection, go straight down to the bottom axis. Read the concentration on the bottom axis. You will need to figure out how far from your known concentrations to get the concentration of the sample. For example, if it falls half way between 25ppm and 50 ppm it is 37.5ppm.If you need more detail there are many sources on-line or in mathematics textbo


What is the consumable that the customer needs to purchase after sometime?

They will probably want our Fluids Kit that contains concentrated calibration standards, diluent concentrate, and Decon 90 cleaning concentrate. Individual solutions are also available. We also have available an Annual Service Kit and a Preventative Maintenance Kit.

How do I clean a BWB flame photometer? My flame photometer was supplied with DECON 90 cleaning solution. Is this the only product I can use to clean the instrument?

The Decon 90 is a non-ionic surfactant. Other cleaning products will work as long as they are non-ionic. The ionic types usually have a Na salt of various aliphatic sulfonates or sulphates. When trying to clean them with ionic types, the Na deposits on the inside surfaces and makes it dirtier! The non-ionic types use ethoxylated alkyl phenols, fatty acid amides, or polymers of oxides and thus have no ions to accumulate inside the burner/mixing chamber.

How do I set up the nebuliser on my new BWB Flame Photometer?

The adjustment on the nebuliser should never be changed. At the present time it is too difficult to explain the process to re-set it. We are working on a method for users but it is not ready. If it has been changed it is best to replace it.

Please explain in detail what “de-proteinizing solution” and “Decon 90” are?

De-proteinizing solution is any cleaning agent that works well cleaning off proteins. Decon 90 is one of these types of cleaning agents that we provide. Proteins in samples can clog the capillary tube on the nebuliser and, with extended use, form a coating in the mixing chamber and burner tube where they can affect the results.

A customer told me that a DECON 90 cleaning would result in a high Na readout. Is DECON 90 a solution of NaOH?

Yes, it is made of NaOH. It sounds like after using it for the day they aspirate Decon 90 solution. In my experience, that will not clean it out properly. The parts need to be taken off the instrument and cleaned using a brush with stiff bristles, like a tooth brush. Rinse it very well with DI water and allow to dry before assembling. The Decon 90 has many chemicals in it. It should be diluted to 2-5% in DI water. If you want to read more about the Decon 90 their web site is A solution of the Diluent Concentrate can be use at the end of the day for about 20 minutes. It will help to clean out the system but it is not a substitute for thorough cleaning.

How often do I need to clean my BWB?

It depends on the nature of the samples and the frequency of use. Simple low concentration salts samples will not require as much cleaning. Samples with high concentrations and/or proteins or oils will need cleaning quite often. A general rule is, if there are problems getting good readings, the first thing is to suspect the instrument needs cleaning. It helps to aspirate DI water or a solution of the Diluent Concentrate diluted at 1-2% after done with a run of samples.


PC Software: Can the save path of the report can be changed? (Don’t save in disk C.)

The software needs to originally save it where it does. After it saves it there, you can go get it (click on "Reports" button) and transfer it to where ever you want. Another file, to disc, or as an attachment to an email. You can even print it out!

BWB Technologies USA

457 Laurence Dr., #124

Heath, TX 75032 


Providing distribution,installation, training, scheduled maintenance

and service in the USA and Canada

Flame Photometers
Proudly Engineered
and Manufactured
in England

Images are for illustrative purpose only. Specification and contents are subject to change without notice.