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Measurement of Sodium and Potassium in Cheese 1 Dried milk is dissolved in warm water. The test solutions and reference solutions are measured directly following dilution with deionised distilled water. Procedure Thoroughly clean and rinse all glassware with deionised distilled water prior to use. It is recommended that polyethylene is used to ensure that sodium does not plate out onto glassware surfaces. If only glass is available, the sodium determination was carried out rapidly. 1.25grams of cheese was accurately weighed out on a 5-figure analytical balance and recorded for backtracking the concentration to the sample size. The sample was then added to a crucible of appropriate size and 10ml of ~30% sulphuric acid. It was ensured that the crucible was less than half full of both the sample and acid added to avoid boiling sample being spilled as it was heated. The oven was heated to 600 degrees Celsius, and the crucible was weighed on the 5-figure analytical balance. The crucible was then added to the oven and left to ash over the course of 2 hours. The sample was then reweighed and placed back into the oven for 5 minutes. The crucible was then weighed again, if the weight of the crucible has not changed from the previous weight, then the sample was finished with the ashing process, if it had significantly changed the process was repeated until all combustible content of the sample had been removed. The ashed sample was then dissolved in 54ml of DI water with 1ml of 10% HCl. This was then filtered through a funnel and Whatman n.5 filter paper into a 100ml polyethylene volumetric flask and made up to 100ml with DI water. A minimum of 5 standards were produced with a concentration range of 0-100mg/l potassium and sodium, to each standard 10ml of ~30% sulphuric acid was added and 1ml 10% of HCl was added. (If the sample contained a higher concentration than these standards, the sample solution was further diluted to bring the concentration under 100mg/l.) The standard was calibrated starting with the lowest concentration standard, working up the concentration gradient over the 5 standards. The results will not be reliable if this solution contains insoluble particles. If insoluble particulate matter is present in the sample, filter the sample through Whatman No.1 filter paper. The filter paper should be weighed on an analytical balance prior to filtration, and then the solution filtered and the paper dried in an oven at low temperature then reweighed and the difference calculated between the pre and post filter paper. This should then be accounted for when calculating concentrations by weight. Preparation of Standard Graph Set the flame photometer in accordance to MultiPoint/Single Ion Calibration found on page 24 of the BWB Technologies Installation and Operation Manual, to measure potassium emission. Nebulise the working standard solutions and adjust the controls until steady zero and maximum readings are obtained. Nebulise the intermediate working standard solutions and construct a graph relating raw emission data (known as RAW in BWB the flame photometer) to concentration of all the standard solutions. 1 AOAC International, ‘AOAC Official Method 990.23: Sodium and Potassium in Dried Milk’, AOAC International (2008), 33.5.12.

About BWB Technologies USA - The Flame Photometer Experts Pushing the boundaries of flame photometry What can be achieved with a flame photometer has been redefined by BWB Technologies USA LLC. With unrivaled accuracy, low cost and ease of use, the BWB flame photometer is not only the best flame photometer available, it is the first real alternative to AAS (atomic absorption spectrometry) and ICP (induced coupling plasma) for measuring Lithium (Li), Sodium (Na), Potassium (K), Calcium (Ca) and Barium (Ba). BWB Technologies USA is an American-owned, exclusive distributor of the award-winning BWB Technologies UK Flame Photometer. Drawing on an Anglo-American team of leading industry specialists we strive to create high quality, cost effective products that redefine what is achievable with a low temperature flame. From our manufacturing plant in Newbury, England, BWB Technologies USA has introduced a series of flame photometer products, and accessories, which exceed existing expectations in terms of specification, usability, accuracy, reporting, build quality and value for money. About Us It has long been our mission to redefine the boundaries of Flame Emission Spectroscopy. With a product that delivers unsurpassed performance and our team of experts available 24/7 for technical support, the BWB Flame Photometers do just that...just add gas. Printer option for all instruments Leading design and development Bold and striking colours Printer option for all instruments 1/13 A flame photometer better than ICP and AAS? We were not content to build an instrument that was just better than the other flame photometers - the BWB flame photometer is a cost effective, accurate and reliable alternative to other technologies such as Inductively Coupled Plasma, ICP and Atomic Absorption Spectrophotometers (AAS). 80% of our customers already own an AAS or ICP, but choose to measure Lithium, Sodium, Potassium, Calcium and Barium with their BWB flame photometer rather than the more expensive and complex AAS or ICP. Accessories, Consumables and Spare Parts- Fast Delivery and Fulfillment! BWB Technologies USA provides quick delivery of you order. Typically you will receive your order within 7-10 days - much faster than any of our competitors! BWB Technologies USA LLC- Available 24/7 A BWB Flame Photometer is an important and necessary instrument in your laboratory and we pride ourselves on delivering first class customer support. Our customer service line is open 24/7 and you can always speak to a REAL person! Normally we will answer all questions at the first contact, but if we need to call you back we will do so within 24 hours! • To contact BWB Technologies USA LLC Customer Support email admin@bwb-america.com • For technical assistance email technicalsupport@bwb-america.com • Or call us toll-free at 1-800-608-9870

Measurement of Potassium and Sodium in Meat 1 Several different techniques can be used for sample digestion and extraction of sodium and/or potassium: oven ashing, perchloric acid ashing, extraction by boiling and acidification with HNO3 as well as extraction by homogenisation in 2% trichloroacetic acid (TCA). Results of this investigation indicated that the TCA extraction method was repeatable and simple. The recovery of known amounts of potassium showed the method to be accurate. The results for sodium showed more variability and gave a value lower than expected by about 5%. The amount of potassium found in pork and lamb ranged between 1750 and 3070ppm and the amount of sodium to be between 350 and 700 ppm. Procedure All solutions are prepared using deionised distilled water unless prompted otherwise. A sample weighing between 1.5 and 3.5grams of tissue is homogenised in 150ml of 2% trichloracetic acid (TCA) and stored overnight at 5ºC in polypropylene containers to avoid sodium plating out onto glassware. The homogenised sample is filtered through Whatman number 40 filter paper into clean polyethylene bottles. A 5ml aliquot of the sample is made up to 15ml with 2% TCA. This solution is then used for sodium and potassium determination. Standards are then made for both sodium and potassium around the expected result. If unsure about the range in which the sample will be read at, the BWB Technology offers a single calibration point analysis. This can then be used to estimate an expected range of results and calibrated after this initial estimation of sample. Standards should be aspirated in order of lowest to highest to avoid previously ran standards contaminating the further calibration standards. It should also be noted that it is of vital importance that further sample dilution is required if the concentration is coming out at over 100ppm. Whilst the BWB Flame Photometer is able to detect ion concentrations over 100ppm, interference begins to become significant in the readings over 100ppm as well as matter being deposited onto the nebuliser array which can be released in later aspirations increasing signal noise and data stability. When Calibrating, all standard solutions should be made up with 2% TCA solution as well as a solution of 2% TCA ran as a blank to reduce chemical interferences. The blank can be run when prompted on the full range of BWB flame photometers. Chemical interference for sodium readings can be reduced by neutralising the solution with a few drops of base solution, however it should be taken into consideration that the base selected for neutralisation should not contain any ions that can contaminate readings. If a base is used to neutralise the solution it should be added to all standards and blanks.2 Preparation of Standard Graph Set the flame photometer in accordance to MultiPoint/Single Ion Calibration found on page 24 of the BWB Technologies Installation and Operation Manual, to measure potassium emission. Nebulise the working standard solutions and adjust the controls until steady zero and maximum readings are obtained. Nebulise the intermediate working standard solutions and construct a graph relating raw emission data (known as RAW in BWB the flame photometer) to concentration of all the standard solutions. 1 Kirkton, A.H. & Pearson, A.M., ‘Comparison of Methods of Measuring Potassium in Pork and Lamb and Prediction of Their Composition from Sodium and Potassium’, J. Anim Sci, 22, (1963), p.125-131. 2 AA Perkin. (1996). Analytical Methods for Atomic Absorptions Spectroscopy. page 30.

Customer support long after the sale I nstallation, training, and scheduled maintenance available anywhere in the USA and Canada Offering the best customer support in the industry BWB Technologies USA LLC is proud to be the exclusive distributor of the award- winning, market-leading BWB Flame Photometers in both the USA and Canada. We pride ourselves on providing the best service and support to our customers, long after we make the sale. Expert advice from technical specialists The majority of flame photometers produced by other manufacturers generally provide poor technical and product support. BWB Technologies USA LLC has demonstrated that we have the staff, infrastructure and technical knowledge to answer your questions FAST! Deal direct, not with a catalog supplier When you buy from BWB Technologies USA you are dealing direct! Unlike catalog companies with thousands of products and limited or no skilled staff, BWB Technologies USA provides product experts who are available to help you. Our phones are manned 24/7. This means that you when you call us you will always speak to a REAL person not a recording. There may be infrequent occasions that our sales or support staff are unable to take your call because they are busy assisting other customers. Our modern telephone system immediately transmits an email to the right individual who will contact you as soon as they are available. Extensive internet knowledge base and video library With a full library of training videos and information available on our site is a valuable resource both before and after you purchase your flame photometer. Click here to see our video library.

The BWB SUGAR Flame Photometer For Fertilizer and Mineral Mining Applications In keeping with the Globally accepted published papers from 1977 forward, potassium and sodium detection is now available online 24/7 with a Uniquely configured channel of detection for extreme accuracy along the range of potassium control. This winning proprietary format has been improved with a unique adaptation and optimisation of the proprietary BWB optical array detection systems, which in this iteration of the BWB Specialty Flame Photometers allows for a single channel of precise and accurate potassium and sodium measurement. This Specialty Flame Photometer has been requested and designed for the global Sugar Production marketplace. There were a few traditional Flame Photometer manufacturers over the decades who had served this market and now these robust and fantastic units are coming out of service. Enter the BWB SUGAR Flame Photometer with specialized potassium and sodium channel detection, supplied with hybridized specific needs multiple point calibration solutions for potassium and sodium. Per our customers’ requests, our Collection Cup provides Real Time sample access for our mixing chamber/detection systems and only requires that the sample be at or near ambient temperature and customer provided ~5ml/minute feed to our unique “Collection Cup” system. FP/PC APP This totally unique BWB feature allows multiple installations details to be shared via intranet or internet links to provide “Distributed Control” via a single Control room with staff completely remote from the Refinery site itself. For large Sugar Companies with multiple sites around the world, this allows Expertise from a centralized location anywhere in the world with real time virtually limitless data accumulation and analysis. .csv files allow users to construct novel and proprietary Excel spread sheets and to share data quickly and efficiently. FEATURES UNIQUELY HYBRIDIZED BWB dual CHANNEL, FOCUSED FOR HIGH ACCURACY POTASSIUM and sodium MEASUREMENTS “IRS” (INTERNAL REFERENCE STANDARD) AVAILABLE 4-20MA 2 WIRE OUTPUT COMPATIBLE WITH SCADA SOFTWARE LIVE ONLINE MONITORING CONSTANT CONDENSATION STREAM FEEDS WITH USE OF THE PROPRIETARY BWB COLLECTION CUP BUILT-IN AIR COMPRESSOR; PIONEERED BY BWB TECHNOLOGIES IN 2007 AND PROVEN NOW WITH OVER 1000 UNITS IN GLOBAL FIELD USE SOLUTIONS AND LABWARE INCLUDED; “JUST ADD GAS”, THE HALLMARK OF THE USER FRIENDLY BWB FLAME PHOTOMETER (FES) PROGRAM SPECIALIZED CALIBRATION “READY TO USE” SOLUTIONS SUPPLIED AT 1, 5, 10 AND 20PPM USER SELECTABLE DECIMAL PLACES INTUITIVE USER INTERFACE FOR TRUE EASE OF USE DISPLAY PROMPTS STEP BY STEP OPERATION DATA SHARING VIA PC LINK WITH BWB’S FP/PC APP Sample rate 2 – 3.5ml/min Tubing materials Silicone and Tygon® Required desk space for Instrument 50cm (H) x 45cm (W) x 45cm (D)* For Safety reasons the Flame photometer requires 1m of unobstructed space above to allow dissipation of heat from the chimney. Instrument size Sugar Flame Photometer 51cm (H) x 38cm (W) x 41cm (D) (20in x 14in x 16in) Shipping 62cm (H) x 55cm (W) x 47cm (D) (24in x 22in x 19in) Weight Sugar Flame Photometer – 15.3kg Shipping – 25kg Optimal range Single point calibration Na 0.1 – 60ppm K 0.05 – 100ppm Multi Point Calibration Na 0.1 – 1000ppm K 0.05 – 1000ppm Reproducibility <1% Coefficient of variability for 20 consecutive samples over ten minutes at concentrations of 100ppm or less. (After instrument stabilisation). Chemical Interference Na/K/Li = <0.5% to each other when equal in concentration at <100ppm Limit of detection (LOD) and limit of quantification (LOQ) LOD LOQ Na – 0.03ppm Na – 0.1ppm K – 0.02ppm K – 0.05ppm Time to stability Less than 15 seconds after sample is introduced into the flame Ions measured Potassium Sodium Interfaces USB 4-20mA output capable of direct link to SCADA systems Optional integrated printer .CSV and .PDF generated reports and files via FP-PC software Collection Cup providing 24/7 live automated sampling* Recommended minimum warm up time based on ambient temperature 21ºC – 40 minutes Power requirements 100V – 250V AC at 50 or 60Hz automatically selecting Fuel requirement Propane, Butane or Natural Gas* regulated to 19Bar. Flow rate of O.4l/min *with modifications. BWB Technologies recommends either Propane or Butane for optimum results. Readout LCD, four line, alpha numeric, back lit. WHAT’S IN THE BOX PC Leads USB and RS232 Selection of 3 Power cables to suit all regions (4th option for China) Gas Hose Aspiration Kit Manuals covering all aspects of the Sugar FP Quick Start guides Warranty Registration Form Certificates of analysis for all provided Fluids Material Safety Data Sheets for all provided Fluids The FP-PC Software installation USB 1L of BWB recommended cleaning solution 150ml calibration fluids for sodium at 10,000ppm. 250ml calibration fluids of potassium at 1, 5, 10 and 20ppm 100ml conical flask with screw lid 100 disposable sample cups 1L of De-Ionised water 2m of waste tube Set of spare fuses 10 x 10ml pipettes 10 x 1ml pipettes

BWB the Flame Photometry experts offers a no-quibble market leading warranty for our range of flame photometers. The BWB warranty provides you with the following product protection: • Covers all parts and technical assistance for 12 months. • Extended warranty options available. Flame Photometer Warranty Registration To register, please take the time to fill out the information below. First name Last name Company/Organization Email Phone Position Continue

Principle Potassium is extracted from soil with ammonium nitrate. Apparatus Bottles - 125 ml, wide mouth, screw cap. Scales - accurate to 1 DP. Shaking machine with platform - approx. 275 strokes per min, length of travel 25 mm. Reagents Ammonium nitrate, M - Dissolve 400 g of ammonium nitrate in water and dilute to 5 l. Standardization is unnecessary. Method Grind soil and pass through a 2 mm mesh sieve. Transfer 20 g of air dried soil into a bottle and top up to 50 ml of M ammonium nitrate. Cap the bottle and shake on the shaking machine for 30 mins. Filter through a 125 mm Whatman No. 2 filter paper and retain the filtered extract (this sample may also be used for the determination of magnesium; and sodium if carried out immediately or the bottle is stored in a polythene bottle). Carry out a blank determination with ammonium nitrate solution. Determination The concentration of potassium in the extract is determined by flame photometry; significant interference by other elements does not occur. Reagents Potassium working standard solutions, 0-50µg/ml of potassium - Prepare solutions containing 0, 10, 20, 30, 40 and 50 µg/ml of potassium in 1M ammonium nitrate. Preparation of Standard Graph Set the flame photometer in accordance to MultiPoint/Single Ion Calibration found on page 24 of the BWB Technologies Installation and Operation Manual, to measure potassium emission. Nebulize the potassium working standard solutions containing 0 and 50 µg/ml of potassium and adjust the controls until steady zero and maximum readings are obtained. Nebulize the intermediate working standard solutions and construct a graph relating meter readings to µg/ml of potassium in all the standard solutions. Examination of Extract Adjust the flame photometer until the steady zero and maximum readings are obtained with potassium working standard solutions containing 0 and 50 µg/ml of potassium. Nebulize the extract and note the meter reading. Calculation of Results Read from the standard graph the number of µg of potassium equivalent to the meter readings of the sample and the blank determinations. Multiply the difference by 5. The result gives the mg/l of extractable potassium in the soil. Appendix Potassium stock standard solution, 1 mg/ml of potassium - Dry potassium nitrate at 102°C for 1 hr and cool in a desiccator. Dissolve 1.293 g of the dried salt in water and add 1 ml of hydrochloric acid, approx. 36% m/m HCl to reduce the pH and aid dissolution of the sample. Dilute to 500 ml and add 1 drop of toluene. Potassium Extraction from Soil 1

Simple, robust & reliable, the Automated Fluid Handling System works seamlessly with BWB Flame Photometers. BWB customers rely on our instrumentation to run all day processing tens or even hundreds of samples. Until now, this operation has been a manual process requiring and operator to introduce each sample. Many organizations seek greater efficiency and automation of their laboratory processes and BWB has the solution. The new Automatic Fluid Handling System (AFHS), offers fast, reliable and effective automation of the flame photometry process. New “Auto Read” Technology is the foundation for automation The goal of an automated system is to perform tasks accurately, reliably and autonomously without the need for human intervention. By clever software/firmware handling of the input from our multiple channel detectors, stability of readings are analytically determined and no longer a subjective user decision. Printer This ultra reliable thermal printer sits atop the main flame photometer enclosure for footprint reduction in valuable lab space. The printer is supplied with print stock derived from recycled paper, has a 10 year shelf life and comes with soy based ink as standard. The dimensions of the unit were also designed to offer compatibility with standard calculator print paper available globally from any high street. Autosampler The BWB auto sampler is available as an optional extra for all the latest models of the BWB flame photometer. Autodiluter BWB has introduced a 10:1 and 100:1 auto-diluter for the latest models of the BWB flame photometer. FEATURES BUILT AROUND BWB’S PROPRIETARY “AUTO READ” TECHNOLOGY “IRS” (INTERNAL REFERENCE STANDARD) AVAILABLE COMPATIBLE WITH ALL 2014 BWB FLAME PHOTOMETER MODELS 5 USER SELECTABLE TRAY CALIBRATION CORRECTION POINTS USER SELECTABLE DILUTION RATIO PURPOSE BUILT AUTO DILUTER ON DEMAND PRINTING OF KEY ANALYSIS DATA WITH BWB’S INTEGRATED PRINTER A SINGLE SHARED POWER SOURCE FOR THE AUTO SAMPLER AND AUTO DILUTER OTHER DILUTIONS ASIDE FROM THE STANDARD OF 100:1 AND 10:1 ARE AVAILABLE WITH THE SAME AUTO DILUTER. CONTACT US FOR SPECIFIC INFORMATION. UP TO 89 SAMPLES INTERCHANGEABLE SAMPLE TRAY Automated Fluid Handling Systems The next generation of automation Check out the AFHS system in action with the BWB BIO flame photometer Example - BWB BIO with AFHS Technical Data AFHS is available with most BWB Flame Photometers Sample rate 4ml/min Tubing materials Translucent PTFE and Tygon® Amount of fluid used per sample based on dilution ratios, DR1 – 5ml DR10 – 0.5ml DR100 – 0.05ml Specificity Na/K/Li = <0.5% to each other when equal in concentration at <100ppm Required desk space for Instrument 50cm (H) x 65cm (W) x 63cm (D) *BIO Flame photometer with BWB Recommended set up positioning. For Safety reasons the Flame photometer requires 1m of unobstructed space above to allow dissipation of heat from the chimney. Instrument size BIO Flame Photometer 51cm (H) x 38cm (W) x 41cm (D) (20in x 14in x 16in) Auto Diluter 30cm (H) x 30cm (W) x 43cm (D)* (12in x 12in x 17in) Auto Sampler 30cm (H) x 18cm (W) x 11cm (D)** (12in x 7in x 4in) Shipping 62cm (H) x 55cm (W) x 47cm (D) x 2 boxes (24in x 22in x 19in) *Inclusive of Cannula operating arm. ** Excluding Dilution Reservoir. Weight BIO Flame Photometer – 15.3kg Auto Diluter – 3.75kg Auto Sampler – 6.9kg Shipping BIO Flame Photometer – 25kg AFHS unit – 17kg Optimal range Multi-point calibration Na – 0.2 – 300mmol/l* K – 0.2 – 200mmol/* Li – 0.2 – 20mmol/l* Ca – 1 – 20mmol/l* *With required Dilution Ratios Reproducibility <1% Coefficient of variability for 10 consecutive samples within optimal range. (After instrument stabilisation). Limit of detection (LOD) and limit of quantification (LOQ) displayed LOD LOQ Na – 0.0013mmol/l Na – 0.0043mmol/l K – 0.0005mmol/l K – 0.0013mmol/l Li – 0.003mmol/l Li – 0.0072mmol/l Ca – 0.0075mmol/l Ca – 0.025mmol/l Time to stability Less than 15 seconds after sample is introduced into the flame Single sample time With Auto Diluter: 130 Seconds including Sample Preparation, Acquisition and flush Without Auto Diluter: 80 Seconds including Acquisition and flush Amount of samples measured per hour With Auto Diluter: 25 Without Auto Diluter: 40 Ions measured Serum Na and K or Li and Ca Urine Na and K or Li and Ca Dilution reservoir size 2.5L with inbuilt low level control Optional 5L tank available Amount of samples per dilution reservoir 2.5L – 120* 5L – 240* *dependant on idle times between traysand sample set-ups. Amount of samples per tray 89 with additional interchangeable trays to speed up sampling time. Calibration concentrations URINE Point 1 Point 2 Point 3 Na 25mmol/l Na 50mmol/l Na 100mmol/l K 25mmol/l K 50mmol/l K 100mmol/l Li 2.5mmol/l Li 5mmol/l Li 10mmol/l Ca 2.5mmol/l Ca 5mmol/l Ca 10mmol/l Point 4 Point 5 Na 200mmol/l Na 300mmol/l K 150mmol/l K 200mmol/l Li 15mmol/l Li 20mmol/l Ca 15mmol/l Ca 20mmol/l SERUM Point 1 Point 2 Point 3 Na 120mmol/l Na 130mmol/l Na 140mmol/l K 3mmol/l K 4mmol/l K 5mmol/l Li 0.5mmol/l Li 0.5mmol/l Li 1mmol/l Ca 1.5mmol/l Ca 2mmol/l Ca 2.5mmol/l Point 4 Point 5 Na 150mmol/l Na 160mmol/l K 6mmol/l K 7mmol/l Li 1.5mmol/l Li 2mmol/l Ca 3mmol/l Ca 3.5mmol/l Recommended minimum warm up time based on ambient temperature 21°C – 40 minutes Power requirements 100V – 250V AC at 50 or 60Hz Fuel requirement Propane, Butane or Natural Gas* regulated to 19Bar. Flow rate of 0.4l/min *with modifications. BWB Technologies recommends either Propane or Butane for optimum results. Readout LCD, four line, alpha numeric, back lit. WHAT’S IN THE BOX PC Leads USB and RS232 Selection of 3 Power cables to suit all regions Gas Hose Aspiration Kit Manuals covering all aspects of the BIO FP and AFHS Quick Start guides Warranty Registration Form Certificates of analysis for all provided Fluids Material Safety Data Sheets for all provided Fluids IQ, OQ, PQ On-line Documentation. Certificate of Compliance will be issued upon completion of the PQ testing. The FP-PC Software installation CD 1L of BWB recommended cleaning solution Complete set of calibration fluids including Diluent Concentrate 250 x 5ml AFHS screw top sample Vials 100ml conical flask with screw lid 100 disposable sample cups 1L of De-Ionised water 2m of waste tube Set of spare fuses 10 x 10ml pipettes 10 x 1ml pipettes

BWB Flame Photometer Products Pushing the Boundaries of Flame Photometry BWB Flame Photometers have revolutionized the use of low temperature flame analysis. Our flame photometers are easy to use, inexpensive to operate and provide unrivaled accuracy, making them not only the first, but the best alternative to AAS and ICP for measuring Lithium Li, Sodium Na, Potassium K, Calcium Ca & Barium Ba. BWB Technologies USA, is the sole distributor of the BWB Flame Photometers in the United States. Our products are manufactured in England. We continue to develop and enhance a series of flame photometer products and accessories which exceed existing expectations in terms of specification, usability, accuracy, reporting, build quality and value for the money. Flame Photometers A solution for every application At BWB Technologies USA, we offer a wide range of instruments to address the specific needs and applications of our customers. Learn More Automated Fluid Handling System Flame Photometer Automation Automated Fluid Handling System (AFHS) products help increase productivity, automate your analysis and improve a result's accuracy. Learn More Accessories, Consumables & Spares Ensures performance We supply a full range of products to ensure that our flame photometers perform to the highest standards for years to come! Learn More BWB Support Services BWB USA offers our unique online IQ, OQ, PQ system and Provides Installation, Training and initial calibration services and annual PM Service anywhere in the USA and Canada. We hold substantial inventory of consumables and common spare parts and normally ship within 48 hours. Technical support is handled within 24 hours by the Founder and Co-Founder of BWB Technologies on technicalsupport@bwb-america.com

The BWB BIO 943 Flame Photometer For biological applications Purpose Built Flame Photometer with specialized software, firmware and hardware The BWB BIO 943 is an offshoot of the popular BWB BIO. A simplified menu structure and enhanced Li detection make it a welcome addition to the BWB family. The ability to calibrate and measure Sodium, Potassium, and Lithium at the same time with the same dilution (from 1:6 to 1:100) streamlines throughput in many laboratories. We have retained the ability to enter and store two user-defined calibration curves should the user wish to readily measure differing sample types. When connected to a PC with the BWB FP-PC app, the user can create .pdf files, .csv files, data presentations and reports that include all the key analysis data. The FP-PC Software also allows setup of the Automatic Fluid Handling System, AFHS, originally designed to work specifically with the BIO-943 unit. The software enables you to view and edit the calibration curves. The intuitive user-friendly operation of the BWB BIO 943 spans all languages and appeals to all levels of users. The BWB BIO 943 is the must-have instrument in today’s research laboratory. For sites that do not allow PCs or data acquisition in the test lab, the Printer provides the “hard copy” required for QA and QC purposes. The environmentally friendly print ink used by the printer has a minimum character capture life of 10 years. The printer is also compatible with other readily available paper stock. To enhance accuracy and reproducibility there is an option to use either Caesium or Lithium as an Internal Reference System (IRS) that acts as an internal standard to adjust small changes during calibration and measuring. FEATURES SPECIALLY CONFIGURED FOR A RANGE OF USER INTERESTS “IRS” (INTERNAL REFERENCE STANDARD) CAESIUM OR LITHIUM OPTION SINGLE POINT CALIBRATION CORRECTION INTERNAL REFERENCE STANDARDS 5 AND 6 POINT MULTI-ION CALIBRATION STANDARDS FOR TWO SEPARATE CALIBRATION CURVES ANALYSING LI, NA AND K OPERATOR INDEPENDENT DETERMINATION OF RESULTS ADVANCED AUTOMATED DETERMINATION OF RESULTS INTEGRATED PRINTER WITH ENVIRONMENTALLY FRIENDLY INK IQ, OQ, PQ WEB-BASED CERTIFICATION STANDARD ON BIO 943AND AVAILABLE ON ALL BWB FES UNITS NEW Technical Data Sample rate 2-3.5ml/min Tubing materials Silicone and Tygon® Specificity Na/K/Li = <0.5% to each other when equal in concentration at <100ppm Required desk space for Instrument 50cm (H) x 45cm (W) x 45cm (D) For Safety reasons the Flame photometer requires 1m of unobstructed space above to allow dissipation of heat from the chimney. Instrument size BIO 943 Flame Photometer 51cm (H) x 38cm (W) x 41cm (D) (20in x 14in x 16in) Shipping 62cm (H) x 55cm (W) x 47cm (D) (24in x 22in x 19in) Weight BIO 943 Flame Photometer 15.3kg Shipping – BIO AV Flame Photometer 25kg Optimal range Multi-point calibration Na – 0.0043 – 300mmol/l* K – 0.0013 – 200mmol/* Li – 0.0072 – 20mmol/l* *With required Dilution Ratios Reproducibility <1% Coefficient of variability for 20 consecutive samples over ten minutes at concentrations of 100ppm equivalent or less. (After instrument stabilisation). Limit of detection (LOD) and limit of quantification (LOQ) LOD LOQ Na – 0.0013mmol/l Na – 0.0043mmol/l K – 0.0005mmol/l K – 0.0013mmol/l Li – 0.003mmol/l Li – 0.0072mmol/l Time to stability Less than 15 seconds after sample is introduced into the flame Ions measured Na, K, and Li Calibration solutions provided URINE Point 1 Point 2 Point 3 Na 25mmol/l Na 50mmol/l Na 100mmol/l K 25mmol/l K 50mmol/l K 100mmol/l Li 2.5mmol/l Li 5mmol/l Li 10mmol/l Ca 2.5mmol/l Ca 5mmol/l Ca 10mmol/l Point 4 Point 5 Na 200mmol/l Na 300mmol/l K 150mmol/l K 200mmol/l Li 15mmol/l Li 20mmol/l Ca 15mmol/l Ca 20mmol/l SERUM Point 1 Point 2 Point 3 Na 120mmol/l Na 130mmol/l Na 140mmol/l K 3mmol/l K 4mmol/l K 5mmol/l Li 0.5mmol/l Li 0.5mmol/l Li 1mmol/l Ca 1.5mmol/l Ca 2mmol/l Ca 2.5mmol/l Point 4 Point 5 Na 150mmol/l Na 160mmol/l K 6mmol/l K 7mmol/l Li 1.5mmol/l Li 2mmol/l Ca 3mmol/l Ca 3.5mmol/l Recommended minimum warm up time based on ambient temperature 21°C – 40 minutes Power requirements 100V – 250V AC at 50 or 60Hz Fuel requirement Propane, Butane or Natural Gas* regulated to 19Bar. Flow rate of 0.4l/min *with modifications. BWB Technologies recommends either Propane or Butane for optimum results. Readout LCD, four line, alpha numeric, back lit. WHAT’S IN THE BOX USB PC Lead Selection of 3 Power cables to suit all regions Gas Hose Propane Adapter Aspiration Kit Manuals covering all aspects of the BIO 943 FP Quick Start guides Warranty Registration Form Certificates of analysis for all provided Fluids Material Safety Data Sheets for all provided Fluids The FP-PC Software installation USB 1L of BWB recommended cleaning solution Complete set of calibration fluids including Diluent Concentrate 100ml conical flask with screw lid 100 disposable sample cups 1L of De-Ionised water 2m of waste tube Set of spare fuses 10 x 10ml pipettes 10 x 1ml pipettes

The BWB SOIL Flame Photometer Highly accurate and cost effective analysis in the lab or 'on the road' Potassium is essential to plant growth, fertilizers and soil substrates have been analysed using flame photometry for decades. Building upon 2017 research papers conducted by Professor Akiharu Sasaki for the specific use of flame photometer analysis in the agricultural industry over and above other methods of analysis the BWB SOIL Flame Photometer was born. This unique instrument has been designed and developed in conjunction with various field requests and operational requirements for the specific analysis of fertilizers and soil substrates. Building upon the footprint of the award winning BWB XP instrument the SOIL offers all the benefits of our base product optimized over and beyond the requirements for analysis in the agricultural industry. The SOIL instrument offers enhanced Ca detection alongside Sodium and Potassium with the option to utilize Lithium as an internal reference standard. The ease of portability of this instrument and ‘Just add Gas’ configuration allows the instrument to be set up in the back of a van and conduct field analysis on site at various geographical locations. With a thermal printer fitted as standard, traceability for sample analysis is maintained throughout. The BWB SOIL is the first instrument of its kind optimized for the agricultural industry. FEATURES SIMULTANEOUS DETECTION AND DISPLAY OF ALL 4 ATOMS OF INTEREST “IRS” (INTERNAL REFERENCE STANDARD) AVAILABLE INTUITIVE USER INTERFACE FOR TRUE EASE OF USE DISPLAY PROMPTS STEP BY STEP OPERATION BUILT-IN AIR COMPRESSOR SOLUTIONS AND LABWARE INCLUDED DATA SHARING VIA PC LINK OPERATOR INDEPENDENT DETERMINATION OF RESULTS 4 USER SELECTABLE UNITS OF MEASURE USER SELECTABLE DECIMAL PLACES INTEGRATED PRINTER USES READILY AVAILABLE PAPER IQ, OQ, PQ WEB-BASED CERTIFICATION AVAILABLE CAN BE USED WITH EITHER THE BWB COLLECTION CUP FEATURE OR OUR AFHS AUTOMATED SAMPLE HANDLING SYSTEM CORRECTION OF CA FOR THE INTERFERENCE FROM HIGH LEVELS OF NA Technical Data Sample rate 2 –3.5ml/min Tubing materials Silicone and Tygon® Required desk space for Instrument 50cm (H) x 45cm (W) x 45cm (D)* For Safety reasons the Flame photometer requires 1m of unobstructed space above to allow dissipation of heat from the chimney. Instrument size Wine Flame Photometer 51cm (H) x 38cm (W) x 41cm (D) (20in x 14in x 16in) Shipping 62cm (H) x 55cm (W) x 47cm (D) (24in x 22in x 19in) Weight Wine Flame Photometer – 15.3kg Shipping – 25kg Optimal range Single point calibration Na 0.1 – 60ppm K 0.05 – 100ppm Ca 1.0 – 100ppm Multi Point Calibration Na 0.1 – 1000ppm K 0.05 – 1000ppm Ca 1.0 – 1000ppm Reproducibility <1% Coefficient of variability for 20 consecutive samples over ten minutes at concentrations of 100ppm or less. (After instrument stabilisation). Specificity Na/K/Li = <0.5% to each other when equal in concentration at <100ppm Limit of detection (LOD) and limit of quantification (LOQ) displayed LOD LOQ Na – 0.03ppm Na – 0.1ppm K – 0.02ppm K – 0.05ppm Ca – 0.3ppm Ca – 1ppm Time to stability Less than 15 seconds after sample is introduced into the flame Ions measured Simultaneous measurement of Na, K, and Ca Interfaces USB 0-2.5 volt output (based on sample concentration linked to element of users choice) Optional 4-20mA output in place of the above Optional integrated printer .CSV and .PDF generated reports and files via FP-PC software Recommended minimum warm up time based on ambient temperature 21ºC – 40 minutes Power requirements 100V – 250V AC at 50 or 60Hz automatically selecting Fuel requirement Propane, Butane or Natural Gas* regulated to 19Bar. Flow rate of 0.4l/min *with modifications. BWB Technologies recommends either Propane or Butane for optimum results. Readout LCD, four line, alpha numeric, back lit. WHAT’S IN THE BOX PC Leads USB and RS232 Selection of 3 Power cables to suit all regions Gas Hose Propane Adapter Aspiration Kit Manuals covering all aspects of the Soil FP Quick Start guides Warranty Registration Form Certificates of analysis for all provided Fluids Material Safety Data Sheets for all provided Fluids The FP-PC Software installation USB 1L of BWB recommended cleaning solution 150ml calibration fluids for all 4 elements at 10,000ppm. 100ml conical flask with screw lid 100 disposable sample cups 1L of De-Ionised water 2m of waste tube Set of spare fuses 10 x 10ml pipettes 10 x 1ml pipettes

BWB Flame Photometers A Solution for Every Application At BWB Technologies USA, we know that our customers expect more than a "one size fits all" approach to Flame Photometry. That's why we developed a full range of instruments to address the needs of multiple industries. We offer you solutions tailored to your specific applications. BWB XP General Purpose The award winning, first and only 5-channel digital Flame Photometer with simultaneous detection and display all 5 elements. Learn More BWB BIO For Biological Applications Created for today's research environment. Able to perform routine measures quickly and accurately. Learn More BWB XP Plus Enhanced Purpose 4-channel option that provides more utility unsurpassed levels of accuracy, usability,and reliability while reducing analysis time. Learn More BWB SUGAR For Fertilizer and Mineral Mining Applications Created to measure biological solutions. and modernized for today's research work. Learn More BWB BIO 943 For Biological Applications Created to measure biological solutions. with simplified menu structure a enhanced Li detection. Learn More BWB FLASH The newest feature to flame photometry Fully configurable up to four channel Flame Photometer that detects and displays all calibrated ions at same time. Learn More What’s in the box Why buy from us instead of a Catalog Supplier? BWB Technologies is focused on producing the best technology and products in the flame photometer market. The feedback from customers is that the key benefits they see with the BWB are as follows: Features Just add gas We have revolutionized the flame photometry market by offering an instrument with all accessories supplied as standard. This means that the process of ordering a BWB flame photometer is incredibly simple. There are no complex product codes, accessory lists or ordering requirements. When you order a BWB flame photometer it will arrive containing everything you need to begin analysis. Built in Air Compressor We were the first and still the only flame photometer manufacturer to provide a built in air compressor. This greatly reduces noise and laboratory footprint, while increasing instrument stability, accuracy and results reproducibility. Up to 5 channels of simultaneous detection and display The ability to measure and calibrate up to 5 different ions at the same time offer huge advantages over more traditional instrumentation. BWB was the first and for a decade with five channels of detection and is Still the only flame photometer to offer simultaneous detection and display of five channels. Our customers do not need to waste valuable time fitting filters and re-calibrating when a different measurement is required. This saves Operator TIme by up to 66% on normal laboratory analysis. Advanced calibration system The ability to carry out a 10 point calibration, store date and perform multi mode (multiple ions) calibrations reduces analysis time significantly and increases accuracy. Computer connectivity is standard The FP-PC software package has been written with GLP compliance in mind and is included with every purchase of a BWB instrument. The software facilitates the creation of data reports in .pdf and .csv formats. The majority of our users create their Own Excel spread sheets which they intranet inter-company or share via the Internet externally. No Extra Charge- a Feature and Benefit of the BWB Flame Photometer. Ease of use With exceptional ease of use, a clear back-lit LCD guides users through analysis using simple menu options. The display, used in conjunction with a comprehensive membrane keypad ensures that accurate and reliable results can be obtained with minimal training. As modern labs may experience a large turnover of staff, lab managers will appreciate the comprehensive training videos supplied with every BWB instrument. Our training videos are also available online in multiple languages. Visit our website on www.bwb-america.com for the full list of our YouTube channel. Reliable and simple Manipulation of the flame is the only adjustment available to the operator during operation ensuring reliable and accurate measurements every time. Benefits Excellent customer and technical support from our office in Yorba Linda, California . Start up and initial training available anywhere in the USA and Canada.Novel Certificate of Competence via online IQ, OQ, PQ program. Unique in this industry, another BWB Innovative Feature and Benefit. BWB Flame Photometers - As easy as 1,2,3 BWB Flame Photometers - As easy as 1,2,3 1 ACCURATE MEASUREMENTS BWB Flame Photometers measure Lithium, Sodium, Potassium, Calcium & Barium (Li, Na, K, Ca & Ba) with unsurpassed accuracy BWB Flame Photometers - As easy as 1,2,3 2 INDUSTRY APPLICATIONS Cement • Beverage • Pharmacies • Medical Schools • Petrochemical Paper • Fertilizer • Food • Nuclear Power Stations • Salt and Potassium mines • Sugar Industry BWB Flame Photometers - As easy as 1,2,3 3 JUST ADD GAS Your BWB Flame Photometer comes with everything you need, right out of the box. No need to buy expensive extras or vital accessories. All you have to do is just add gas.